高温对淮南麻黄鸡血脑屏障的影响

doi:10.11843/j.issn.0366-6964.2017.04.021

Abstract

Abstract:

The aim of the present study was to explore the impact of high temperature on Huainan partridge chickens’ blood-brain barrier. One hundred and twenty 107-day old Huainan partridge chickens were randomly divided into four groups: chickens in group Ⅰ and Ⅱ were raised at normal room temperature (21-23℃, RH 60%-70%), which in Group Ⅲ and Ⅳ subjected to hyperthermia (34-37℃, RH 45%-50%) for 3 hours daily. The treatment lasted for two weeks. Five chickens were randomly selected from each group on day 7 and 14, respectively, chickens in group Ⅱ and Ⅳ were received a single intravenous dose of Gamma-aminobutyric acid (GABA) (8.87 mg·kg^-1 body weight), chickens in group Ⅰ and Ⅲ received same dose of saline, brain tissue samples were collected at time 0.5 h and analyzed GABA using ELISA assay. The brain tissue samples were collected on day 14, and the ultrastructure of the blood brain barrier were detected using transmission electron microscopy (TEM), the mRNA and protein expression of ZO-1 and Occludin in the brain tissues were detected by qRT-PCR and Western blot. The results showed that, On day 7 and 14, no significant differences of brain GABA concentration were found between group Ⅰ and Ⅱ (P>0.05). Brain GABA concentration of chicks in Group Ⅳ were higher than that in group Ⅲ on day 7 and 14, and the differences were very significant on day 7 (P<0.01). Compared with normal temperature, high temperature destroyed the blood-brain barrier structure of chickens. Mitochondrial cristae of Cerebral cortical capillary endothelial cells were damaged, some broken, some degeneration. Capillary endothelial cell tight junctions gap widened. Microvascular basement membrane became loose and thick. The mRNA transcription of ZO-1 and Occludin were very significantly (P<0.01) and significantly lower (P<0.05) than those in normal temperature group, respectively. The protein relative expression of ZO-1 and Occludin were very significantly (P<0.01) lower on days 14 than those in normal temperature group. High temperature destroyed the chicken blood-brain barrier, ZO-1 and Occludin down-regulation may be one of the mediators of it.

CLC Number:

S852.3

JIANG Dan, FENG Shi-bin, WANG Xi-chun, LI Yu, ZHAN Kai, WU Jin-jie, LI Jin-chun. Effects of High Temperature on Huainan Partridge Chickens' Blood-brain Barrier[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 769-776.

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Effects of High Temperature on Huainan Partridge Chickens' Blood-brain Barrier

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